Photopic flicker optoretinography captures the light-driven length modulation of photoreceptors during phototransduction

In this study, we used an inhibitor of phosphodiesterase 6 (PDE6) to examine the impact of changes in the conformation of the PDE6 protein on the light-induced process responsible for altering the length of the outer segments of photoreceptor cells in both human and rodent eyes. We employed a imaging method called spatiotemporal optical coherence tomography, which ensures high contrast and phase stability within the strongly scattering photoreceptor– Retinal Pigment Epithelium complex. Using this approach, we recorded nanometer-scale changes in human cones and rods in response to photopic flicker stimulation and observed length changes in rodent rods under scotopic conditions following a single pulse of light, in the absence or presence of sildenafil, which inhibits the catalytic activity of PDE6. Our findings are consistent with the interpretation that during phototransduction conformational changes in PDE6 structure, which occur on an angstrom scale, are amplified to the nanometer sca..., , # Photopic flicker optoretinography captures the light-driven length modulation of photoreceptors during phototransduction

**Folder: Figure2.zip contains 1 file:**

**Figure2_data.mat**

-Variable named \"experimentalResults\" contains structs with measurement descriptions and results (cell named “ORG”). \"ORG\" cell contains, ORG response (nm), high-pass filtered response (nm), amplitude spectrum (nm), frequency-axis (Hz), time-axis (s), and finally amplitude (nm) and phase of the spectrum at stimulus frequency.

-Variable \"t\" contains the time axis (s).

-Variables \"ORG*\" contain recorded ORG signals (nm) at given stimulus values.

-Variables \"*Comp*\" contain extracted components (\"slow\", \"fast\", \"flicker\") with values given in nm at different stimulus luminances.

 

**Folder: Figure3.zip contains 1 file:**

**Figure3_data.mat**

-Variables named \"subject (1-6)\" contain measurement results for different subjects. In each of the structs, there are measurement descriptions and results (ce...,