Expression of candidate genes by keratinocytes co-cultured with skin commensal. Expression of candidate genes by keratinocytes co-cultured with skin commensal

To investigate whether skin bacteria might influence the expression of selected genes, we co-cultured human keratinocytes with S. epidermidis, an abundant commensal in human skin and performed RNA sequencing analysis. Overall design: Keratinocytes were cultured with and without the addition of Staphylococcus epidermidis cultures, six replicates in each group. Host RNA were isolated and RNA-Seq was performed to identify differential expression of selected genes.