Total differential RNA-seq in the marine proteobacterium Vibrio harveyi

Objectives: determination of transcription start sites in Vibrio harveyi genome and discovery of new transcripts Methods: we performed differential seqencing of total RNA isolated from o.n. control Vibrio harveyi cultures. Sample treatment with Terminator EXonuclease (TEX) allowed differenciation of primary and secondary transcripts, helping in the definition of transcription start sites (TSS) Results: by data-mining RNA-seq data and performing some Northern Blot experiments we were able to detect new putative small-RNAs, along with these results, a more deep analisys of our RNA-seq data will give futher insight into genetic organization of Vibrio harveyi genome to help in its investigation Overall design: Total RNA was isolated from control o.n. cultures of Vibrio harveyi cells, strain ATCC 14126, by triplicate. RNA samples were differentialy treated with TEX and sequenced. Reads were aligned to Vibrio harveyi strain ATCC 43516 to detect TSS contributor: Vertis Biotechnologie AG