Pre-existing Dual Epigenetic Barriers Define Heterochromatin Boundaries in Plants

1. Rice heterochromatin boundaries identified in Fig. 1b. 2. Rapeseed heterochromatin boundaries identified in Fig. 1c. 3. Arabidopsis ROS1-maintained accessible chromatin boundaries identified in Fig. 2a. 4. Rapeseed ROS1-maintained accessible chromatin boundaries identified in Fig. 2c. 5. NRPD1-dependent heterochromatin spreading sites identified in Fig. 2e. 6. DDM1-regulated accessible chromatin boundaries identified in Fig. 3a. 7. DDM1-regulated accessible chromatin boundaries in ros1-4 background identified in Fig. 3c. 8. HDA6-regulated histone acetylation boundaries identified in Fig. 4a. 9. Histone acetylation-associated heterochromatin boundaries across 20 rice accessions identified in Fig. 5a. 10. Dual epigenetic boundaries of Type 1 across 20 rice accessions identified in Fig. 5d. 11. Dual epigenetic boundaries in Arabidopsis identified in Fig. 5g. 12. Pre-existing boundaries that prevent transposon insertion-induced heterochromatin invasion across rice accessions identified in Fig. 6a.