In order to establish the genome editing tool, we selected two target sites respectively on pyrE gene. Transformants on the plates were randomly selected and incubated in the liquid medium. The target
Purpose: The goals of this study are to introduce a new genome editing tool, which has the higher editing scope than the original genome editing tools. Methods: First, we transfected PE2 (the original
The table depicts the gene-targeting efficiencies into two independent genetic loci of WT and Δxpf cells. For each locus multiple transfections were performed and drug-resistant clones were analysed b
