Unexpected nuclear hormone receptor and chromatin dynamics regulate estrous cycle dependent gene expression [HIF2A cKO RNA-seq]

Uteri from intact, untreated CD-1 mice were collected at 2 months of age at diestrus (CoD) or estrus (CoE). RNA-seq, ATAC-seq, ER alpha ChIP-seq, PgR ChIP-seq, Hif2a ChIP-seq and HiC-seq were performed as well as RIME assay using ER alpha as the immunoprecipitating antibody. Uteri from intact, WT and HIF2A cKO untreated FVB/N mice were collected at 2 months of age at diestrus (CoD) or estrus (CoE). RNA-seq was performed.本数据集包含两组小鼠子宫组织的组学与分子实验数据： 第一组样本来源于生殖系统完整、未接受任何处理的CD-1小鼠，于2月龄时分别在动情间期（diestrus，缩写CoD）与动情期（estrus，缩写CoE）采集子宫组织。针对该组织样本，我们开展了RNA测序（RNA-seq）、转座酶可及性测序（ATAC-seq）、雌激素受体α（ER alpha）染色质免疫共沉淀测序（ChIP-seq）、孕激素受体（PgR）ChIP-seq、缺氧诱导因子2α（Hif2a）ChIP-seq及Hi-C测序（HiC-seq），同时以ER alpha作为免疫沉淀抗体完成了RIME测定。 第二组样本来源于生殖系统完整、未接受处理的野生型（WT）及HIF2A条件性敲除（cKO）FVB/N小鼠，于2月龄时分别在动情间期与动情期采集子宫组织，仅对该样本开展了RNA测序。