Highly accurate Skin-Specific methylome analysis algorithm as a platform to screen and validate therapeutics for healthy aging [DS2]

In the present study, we developed a highly accurate Skin-Specific DNAm age predictor, using DNAm data obtained from 508 human skin samples. Based on the analysis of 2,266 CpG sites, we accurately calculated the DNAm age of cultured skin cells, and human skin biopsies. Age estimation was sensitive to the biological age of the donor, cell passage, skin disease status, as well as treatment with senotherapeutic drugs. Analyze the effect of cell culture (cell passage) on the methylation profile. Sample 1: human progeria dermal fibroblast at passage 11 in triplicate Sample 2: human progeria dermal fibroblast at passage 19 in triplicate