Results of in silico analysis using SignalP, SecretomeP and TMHMM servers predicting the localization of identified proteins.

a) The presence of signal peptides in a protein sequence was predicted using SignalP 4.0. A protein was classified as being classically secreted when it returned a D-score exceeding 0.450 (indicated by bold numbers).b)For the prediction of proteins secreted via non-classical secretory pathways, SecretomeP 2.0 was used. Proteins win an N-N score exceeding 0.500 (indicated by bold numbers) were predicted to be secreted.c)Prediction of transmembrane helixes was performed using TMHMM v2.0. A protein with its expected number of amino acids in transmembrane helixes (ExpAA) exceeding 18.000 (indicated by bold numbers) was predicted to be a plasma protein.To be classified as “secereted”, a protein must fulfil at least one of the following criteria: It is predicted by SignalP to possess a signal peptide, it is predicted by SecretomeP to non-classically secreted, or, TMHMM predicts that it possesses a transmembrane helix. All proteins, with the exception of aldose reductase (marked with a #), were found to fulfil at least one of these criteria.Results of in silico analysis using SignalP, SecretomeP and TMHMM servers predicting the localization of identified proteins.