N6-methyladenosine (m6A) modification in HaCaT cells (immortalized human keratinocyte cell line) treated with STM2457 in the presence or absence of M5 (inflammatory factor cocktail) stimulation [MeRIPseq_KC]

We conducted an analysis of N6-methyladenosine (m6A) modifications in HaCaT cells, an immortalized human keratinocyte cell line, following treatment with METTL3 inhibitor, STM2457, under conditions of either M5 (inflammatory factor cocktail) stimulation or its absence. The primary aim of this investigation was to establish a molecular framework elucidating the role of m6A modifications in modulating the functional dynamics of keratinocytes within an inflammatory milieu. Overall design: HaCaT cells were subjected to a 24-hour treatment regimen with the METTL3 inhibitor STM2457, succeeded by a subsequent 24-hour exposure to either M5 or phosphate-buffered saline (PBS). Each experimental group comprised two replicates.