Inula japonica Random survey. Inula japonica

Inula japonica, Inula hupehensis and Inula linaariifolia are all medicinal plants of Inula. L in Compositae, and Inula hupehensis is endemic to China. In order to compare the genomic sequence differences of three species and provide scientific basis for their germplasm resources conservation and development, we obtained and analyzed the complete chloroplast genome of three species of Inula L. s. Total DNAs were extracted from the fresh leaves and subjected to next-generation DNA sequencing. NOVOplasty was used to assemble the chloroplast genome from the sequence reads. Then, CPGAVAS2 was used to annotate the genes and repeats in the genome. Lastly, phylogenomics analysis was conducted using RAxML. The results showed that the total length of the chloroplast genome of Inula japonica, Inula hupehensis and Inula linaariifolia were 150754bp, 150 909 bp, and150812bp, consisting of a large single copy region (LSC), a small single copy region (SSC) and a pair of inverted repeats (IRs) regions. In addition, the G/C content of three chloroplast genome were almost 37.7% and they all encoded 111 unique genes, including 79 protein-coding, 28 tRNA and 4 rRNA genes. Meanwhile, 32,33,34 simple repeat sequences, 18,22,18 tandem repeat sequences and 33,37,38 scattered repeat sequences were identified in three species. Phylogenomic analysis showed that all three species of Inula L. and C. bracteatum were clustered together, the relationship between Inuleae and Senecioneae is closer, which suggest that Inuleae may originated from the Senecioneae, not the Cardueae. All data in this study not only enriched the chloroplast genome database of Inula, but also laid the foundation for the future studies of species identification, phylogenetic relationship, evolution history and genetic diversity of Inula species.