Claudin-1 as a novel target gene induced in obesity and associated to inflammation, fibrosis and cell differentiation

T-lymphocytes from visceral and subcutaneous white adipose tissue (vWAT and sWAT, respectively) can have opposing roles in the systemic metabolic changes associated with obesity. However, few studies have focused on this subject. Claudin-1 (CLDN1) is a protein involved canonically in Tight Junctions (TJs) and tissue paracellular permeability. We evaluated the gene expression in T lymphocytes from vWAT and sWAT and in the whole adipose depots. A Clariom D-based transcriptomics analysis was performed in T lymphocytes magnetically separated from vWAT and sWAT from patients with obesity (Cohort 1; N = 11). The experimental design for hybridization processing included 4 batches of 9-11 samples each with balancing among different batches between the two compared conditions: vWAT and sWAT.