RNA sequencing analysis of embryonic yolk sacs from wildtype and Etv2 mutant embryos at E8.5

Transcriptomic analyses of yolk sacs from mouse embryos at E8.5 was performed to assess the dosage dependent effects of varying Etv2 dosage on early endothelial and hematopoietic development. Overall design: Mouse embryonic yolksacs were collected across 6 different Etv2 genotypes with varying Etv2 dosage levels from 100% to 0%. Samples were somite-matched and littermates were included as controls. Samples from both sexes were used in the analysis. 4-6 biological replicates were used per group.