TARGET-Seq: A Protocol For High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing
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资源简介:
TARGET-Seq is a protocol for TARGETed high-sensitivity single-cell mutational analysis, parallel RNA SEQuencing and cell-surface proteomics, with extremely low allelic-dropout rates . In this manuscript, we present a detailed step-by-step protocol for the application of TARGET-seq (Rodriguez-Meira et al, Molecular Cell, 2019), including troubleshooting tips, approaches for automation and methods for high-throughput multiplexing of libraries. In this dataset, we provide program files for specific liquid handlers corresponding to each step of the protocol.
创建时间:
2020-09-10



