Data and code from: Cholecystokinin modulates age-dependent thalamocortical neuroplasticity

The thalamocortical pathways exhibit neuroplasticity not only during the critical period but also in adulthood. In this study, we investigated how cholecystokinin (CCK) modulates age-dependent thalamocortical plasticity. Our findings demonstrated that CCK is expressed in thalamocortical neurons and that high-frequency stimulation (HFS) of the thalamocortical pathway triggers the release of CCK in auditory cortex (ACx), as detected by a CCK sensor. HFS of the medial geniculate body (MGB) induced thalamocortical long-term potentiation (LTP) in wild-type young adult mice. However, knockdown of Cck expression in MGB neurons or blockade of the CCK-B receptor (CCKBR) in the ACx abolished HFS-induced LTP. Interestingly, this LTP could not be elicited in juvenile (3-week-old) or aged mice (over 18-month-old) due to distinct mechanisms: the absence of CCKBR in juveniles and the inability to release CCK in aged mice. Notably, exogenous administration of CCK into the ACx rescued LTP in aged mice a..., , # Dataset README

> Dataset DOI: [10.5061/dryad.k0p2ngfmt](https://doi.org/10.5061/dryad.k0p2ngfmt)\
> Title: **Cholecystokinin gates age-dependent auditory thalamocortical plasticity**\
> Date: **2025-10-02**\
> Version: Data archive (**2025.7z**), Code archive (**matlab_script_for_data_analyze.zip**)

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## Overview

This repository contains all experimental data and analysis scripts underlying the figures and results, including:

* In vivo electrophysiology (fEPSPs)
* Fluorescence signal traces
* Behavioral data from prepulse inhibition (PPI) acoustic startle tests
* Immunohistochemistry (IHC) quantification

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## Contents and Structure

### 1) Data archive: **2025.7z**

Includes raw and processed data for all experiments:

* Electrophysiology recordings: baseline and post-manipulation segments
* Fluorescence signal traces with time axis
* PPI acoustic startle behavioral tables
* IHC quantification tables

### 2) Code archive: **matlab_script_for_data_analyze.zip**

MATLAB scrip...,