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Iberian Hare gut microbiota amplicon metagenome in areas of different agricultural management regimes Raw sequence reads

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP536568
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Wild populations of Iberian hare inhabiting organic (pesticide-free management) and conventional (pesticide-intensive) farming fields in the center of Spain were studied by massive 16S rRNA gene sequencing of duodenum, caecum, and rectum tissues and fresh feces, respectively. Hares were harvested by legally licensed volunteer hunters during their regular hunts in 2018-19 hunting season and donated for this study. The authors declare that this study is based on legal hunting methods according regional laws. For each hunted individual a intestine tissue section of duodenum, caecum, rectum and fresh feces were respectively collected and conserved with lysis buffer (40 mM EDTA; 50 mM Tris pH 8.3; 0.75 M sucrose) at -80 Celisius degrees until DNA extraction. Overall, 148 samples were initially analyzed. Raw sequences are from 16S rRNA amplicon sequencing (hiper-variable V4 region).
创建时间:
2025-01-12
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