Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:774, Patiria miniata, sperm. CIL. Dataset

Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 3 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 160, 120, and 104eV (this image).

磷元素具有特征性的电子能量损失，其边缘位于160 eV。蔡司902型电子显微镜允许记录不同能量损失下的图像，这些图像可数字化处理，从而生成净磷图像。对于核染色质，磷主要位于DNA中，这使其在染色质纤维中的分布得以绘制。电子能量损失成像（EELS）亦被称为电子能谱成像（ESI）以及电子能量过滤透射电子显微镜（EFTEM）。海星精子染色质，具有清晰定义的染色质纤维，经过低温固定、Lowicryl介质中包埋，制备了薄片并进行了无染色的成像。三组图像使用蔡司902型能量过滤透射电子显微镜在80KV电压下，以5eV的窗口，分别聚焦于160eV、120eV和104eV的能量损失值处进行记录（此图像）。