RNA-sequencing of soybean cyst nematode induced response in Glycine soja and Glycine max

RNA-seq based transcriptomes of soybean cyst nematode (SCN) resistant wild soybean (Gylcine soja), NRS100 (nematode resistant soja) with three other genotypes: susceptible wild soybean genotype S-soja (PI468396B), resistant Glycine max cultivar Peking (PI548402), and susceptible Glycine max cultivar William 82 (PI509044), were generated to understand the unique resistance mechanism in NRS100. NRS100 is significantly resistant (Female Index = 3.3) to SCN HG Type 2.5.7 (SCN race 5), which is prevalent in the south-eastern United States. RNA of each genotype was collected in triplicates for two conditions: control and SCN treated. Each replicate was run on two separate lanes in HiSeq. These RNA-seq transcriptomes of SCN treated and control conditions across four genotypes: Peking , Williams 82, S-soja, and NRS100 are used to determine differential regulation between genotypes. Nematode susceptible and resistant genotypes from both cultivated and wild soybeans are used to elucidate species-specific and genotype-specific responses.