GNPS - Guarana_extratos_modo_negativo_FEV_JNA

The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil. The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis. The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode.
Luna5uPFP2;100A_15X2
Flx=0,1875 mL/minSSplit; P:1026 psi;F:30C;
C=1mg/mL(MeOH/H2O_1:1_0.1%ACF);Inj=10uL;
A(H2O+0.1%ACF)/B(ACN+0,1%ACF)HONEYWELL06/02
0-60_8-22%
60-62m_22-8%
62-70m _8%
injetor: MeOH/H2O_(0.1%ACF_1:1) (06-02-23)
Calib.:HCOONa 10mM_end