Characteristics of mosquito stage specific proteins selected on the basis of expression pattern during sporozoite development.

1Genes shown in bold been further characterized by functional analysis through targeted gene disruption (see Table 4).
2Classification of proteins based on their protein expression patterns: group I (ODS-enriched), group II (SGS-enriched), and group III (ODS/SGS-enriched).
3Abbreviations used for motif predictions: are Signal Peptide (SP), Glycosylphosphatidylinisotol anchored proteins (GPI), Trans Membrane regions (average of number of TM regions predicted by four algorithms) and N-terminal PEXEL (Plasmodium export element within first 200 amino acids obtained from http://www.plasmodb.org). Abbreviations for predicted SMART domains: Apical membrane antigen (AMA), Complement control proteins (CCP), Domain of Unknown Function (DUF), Glu/Leu/Phe/Val dehydrogenase dimerisation domain (ELFV_dehydrog_N), Glutamate/Leucine/Phenylalanine /Valine dehydrogenase (ELFV_dehydrog), Domain containing 5 cysteine conserved residues (GCC2_GCC3), Metal dependent phosphohydrolases with conserved ‘HD’ motif (HDc), Membrane-attack complex /perforin domain family (MACPF), Phosphoenolpyruvate carboxykinase (PEPCK_ATP), Papain family cysteine protease (Pept_C1), Sexual stage antigen s48/45 domain (s48-45), Thrombospondin type 1 (TSP1), Triose-phosphate Transporter family (TPT), UAA transporter family (UAA), Von Willibrand factor type A domain (VWA).
4Orthology predicition from OrthoMCL database (http://www.orthomcl.org) shows OrthoMCL group, nr species Plasm (the number of Plasmodium species, additional nr species Api (number of additional Apicomplexan species without Plasmodium species), additional species other (number of species without Apicomplexan species (including Plasmodium)).
5OOC – oocysts; ODS - oocyst-derived sporozoites; SGS - salivary gland sporozoites. The mosquito fraction (mosq fr.) of a protein is calculated by the number of detected peptides in mosquito stages divided by the sum of the number of peptides in mosquito and blood stages.