XIST and MUC1-C form an autoregulatory pathway in driving cancer progression

MUC1-C /XIST autoregulatory axis is of importance in cancer progression where MUC1-C regulates XIST lncRNA levels in cancer RNA Seq in DU145 prostate cancer cell line with tet-XIST shRNA (from GenePharma (Shanghai, China)) silencing. Transduced cells were selected for growth in 1-2 g/ml puromycin or 100 g/ml geneticin. For inducible gene silencing, cells were treated with 0.1% DMSO as the vehicle control or 500 ng/ml doxycycline (DOX; Millipore Sigma).