Pangenomic HCV RNA detection.
收藏Figshare2022-10-25 更新2026-04-28 收录
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Viral RNA from the six HCV genotypes at various input copy numbers were amplified via two-step RT-RPA. Genotyped viral RNA was extracted from HCV-positive human serum and spiked into reverse transcription and RPA reactions for detection. The lowest LODs were observed for genotypes 1 and 4. Genotypes 3 and 6 were the most challenging to detect with inconsistent amplification observed at 100 copies per reaction or less. All no template controls (NTCs) did not amplify in RPA (N = 28).
创建时间：
2022-10-25
