MiR-107 was involved in Lin28-mediated chemo-drug resistance.

(A).RNA level of Lin28 and miR-107 were detected by qRT-PCR in gastric cell line. (B).MiR-107 expression level were detected by qRT-PCR after Lin28 overexpression (in MKN45 and MKN28) or Lin28 knockdown (in MKN45/Lin28). (C). Pre-miR-107 transfection down-regulated Lin28 in MKN45/Lin28 in a time-dependent manner. (D). Chemo-drug sensitivity of MKN45/Lin28C2 and MKN28/Lin28C3 increased after transfection of pre-miR-107. (E). Apoptosis rate of MKN45/Lin28 (treated with OXA at 3.2ug/ml) increased after transfected with pre-miR-107. (F). Chemo-drug sensitivity of MKN45 cell changed after transfected anti-miR-107. (G) and (H). P-gp, C-myc expression were decreased, while CyclinD1 expression was increased in MKN45/Lin28 cells after transfected with pre-miR-107.Each data point represents the mean±SD of three independent experiments. (**p<0.01,*p<0.05).