Next Generation Sequencing Facilitates Quantitative Analysis of young nand aged control and Acsl6-/- Transcriptomes
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived cerebellar transcriptome profiling (RNA-seq) to determine age-relatined impact of acsl6 deficiency on brain transcriptome profiles.Methods: Cerebellar mRNA profiles of 2-month and 18-month control and long-chain acyl-coa synthetase 6-knockout (Acsl6) mice were generated and sent to Genewiz for RNAseq analysisResults: Using an optimized data analysis workflow, we mapped about 3,451,694,754 sequence reads total and ~25-45 million reads per sample of which ~98% were mapped to the mouse genome.Conclusions: Our study represents the first detailed analysis of cerebellar transcriptomes of Acsl6 knockout mice, compated to controls, at young and old ages.
提供机构：
Department of Physiology at the Bro
创建时间：
2022-02-20
