m6A mRNA modification is a rheostat to maintain colonic epithelial cell homeostasis [scRNA-seq]

The function of m6A modification in colonic epithelial cells has been validated in our study that depletion of METTL14 in colonic epithelial cells triggers cell death. To further explore the biological effects of m6A deficiency in colonic epithelial cell heterogeneity, we performed single cell RNA-seq of colonic epithelial cells form 2-week-old Mettl14-KO and WT control mice Single Cell Profiles of Mettl14 WT and Mettl14 KO colonic epithelial cells of two weeks本研究证实了m6A修饰（m6A modification）在结肠上皮细胞中的功能：结肠上皮细胞内METTL14（甲基转移酶样14）的缺失可触发细胞死亡。为进一步探究m6A缺失对结肠上皮细胞异质性的生物学效应，我们对2周龄Mettl14基因敲除（Mettl14-KO）小鼠与野生型（Wild Type, WT）对照小鼠的结肠上皮细胞开展了单细胞RNA测序（single cell RNA-seq）。本数据集为2周龄Mettl14野生型与Mettl14基因敲除结肠上皮细胞的单细胞转录组图谱。