Quantitative proteomic analysis of liver proteins from WT and db/db mice

Liver is a key organ which controls glucose and lipid homeostasis. Dysregulation of hepatic glucose and lipid metabolism causes metabolic diseases, including type 2 diabetes (T2D) and metabolic-associated liver diseases (MAFLD). However, how glucose and lipid homeostasis are integratively regulated is not fully understood. The key factors that drive the pathogenesis of metabolic diseases need to be identified. To identify these regulators, we performed quantitative proteomic analysis in the livers of wide type and db/db mice (n=4 for each group). The quantitative proteomic analysis showed that 1233 proteins were upregulated and 2724 proteins were downregulated in the livers of db/db mice. These differentially regulated proteins contribute to the pathogenesis of metabolic diseases.