Validation by Q-PCR of the gene expression profile in MYBBP1A-depleted HeLa cells. #.
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#Real-Time PCR was performed on HeLa control (High-GC) or MYBBP1A-depleted HeLa cells (siRNA1) with the same RNA samples used for microarray experiment. Two biological replicates were used for each condition and the experiment was performed in triplicate; an arbitrary value of 1 was given to the control (CTL).*Ratio between the expression in down-regulated vs. control (see Methods) cells.**p≤0.01.***p≤0.001 (t test).
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2015-12-02
