A FXR/RXRα/CDCA-responsive element is located in the −122/−69 region of the SHP promoter.

A) shows an overview of the different constructs used to localize the FXR-responsive element in the −278/−69 region of the SHP promoter. Relevant binding sites for other NRs are included. (B, C) DLD-1 cells were transfected with the indicated hSHP promoter constructs and expression plasmids for hFXR and hRXRα. Cells were treated with or without 100 µmol/L CDCA (B) or 1 µmol/L GW4064 (C). Luciferase activity was measured to determine SHP promoter activity. Data are presented as means of ± SD; n≥3. Significant differences are indicated when compared to CDCA/GW4064-treated −569/+10 (a); CDCA/GW4064-treated −303/+10 (b); CDCA/GW4064-treated −278/+10(c). P≤0.05 in a pairwise comparison by Mann-Whitney U test.