Data from: Time of day prioritizes the pool of translating mRNAs in response to heat stress

The circadian clock helps organisms to anticipate and coordinate gene
regulatory responses to changes in environmental stimuli. Under growth
limiting temperatures, time of day modulates the accumulation of
polyadenylated mRNAs. In response to heat stress, plants will conserve
energy and selectively translate mRNAs. How the clock and/or time of day
regulates polyadenylated mRNAs bound by ribosomes in response to heat
stress is unknown. In-depth analysis of Arabidopsis thaliana translating
mRNAs found that time of day gates the response of approximately one-third
of the circadian regulated heat responsive translatome. Specifically, time
of day and heat stress interact to prioritize the pool of mRNAs in cue to
be translated. For a subset of mRNAs, we observed a stronger gated
response during the day, and preferentially before the peak of expression.
We propose previously overlooked transcription factors (TFs) as regulatory
nodes and show that the clock plays a role in the temperature response for
select TFs. When the stress was removed, the redefined priorities for
translation recovered within one-hour, though slower recovery was observed
for abiotic stress regulators. Through hierarchical network connections
between clock genes and prioritized TFs, our work provides a framework to
target key nodes underlying heat stress tolerance throughout the day.