Chan-Soo Lee, Chang-Ki Choi, Eun-Young Shin, Martin Alexander Schwartz, Eung-Gook Kim (2011) CIL:26543, Mus musculus, fibroblast. CIL. Dataset

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were treated with βPIX siRNA (Scr) for 2 days, treated with DMSO for 30 min., and stained for βPIX (green) and vinculin (red). In scrambled siRNA-treated cells treated with DMSO, vinculin localized to large focal adhesions at the cell periphery that overlapped with βPIX. βPIX siRNA had little effect on its own. These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is the original data file from Fig. 6A, “Requirement for βPIX in MII-regulated cell protrusion and adhesion.” J. Cell Biol. 2010. Vol. 190(4):663–674.

为探究非肌肉肌球蛋白II（MII）调节迁移细胞突出和粘附动力学的作用机制，NIH3T3细胞经βPIX siRNA（Scr）处理2天，随后用DMSO处理30分钟，并染色以检测βPIX（绿色）和vinculin（红色）。在经随机siRNA处理的细胞中，用DMSO处理后，vinculin定位于细胞边缘的大型焦点粘附区域，该区域与βPIX重叠。βPIX siRNA本身对βPIX的影响甚微。这些发现有助于阐明MII与Rac1/Cdc42 GTPase之间的功能联系，后者可能调节迁移细胞中的突出/粘附动力学。本图是图6A“βPIX在MII调节细胞突出和粘附中的必要性”的原数据文件。J. Cell Biol. 2010. Vol. 190(4):663–674。