Comparative Transcriptomic Analysis of Vascular Endothelial Cells after Hypoxia/Reoxygenation Induction

Hypoxia and re-oxygenation are common features in many physiological and pathological processes such as, inflammation and tumorigenesis. Vascular endothelial (VEC) participates in the regulation of a variety of important balances in the body and the regulation of cellular functions, plays an important role in the reaction of organs such as ischemia, hypoxia, and inflammatory immunity. HUVEC (Procell Life Science ＆ Technology Co,. Ltid.) were replaced with fresh medium at 37℃ in a humidified atmosphere of 5% CO2 and 95% air which the O2 concentration was about 20% for 12 hours before exposure to hypoxia. Hypoxia cultures (5% O2) were incubated under 5% O2, 5% CO2 and 90% N2 by 3131 Single Tri-gas 184L incubator (Thermo Fisher scientific, Shanghai, China). Hypoxia/re-oxygenation group HUVEC were incubated under hypoxia for 3 hours followed by 1 hour 95% air/5% CO2. HUVEC cultured only in 95% air/5% CO2 were used as the normoxia control group.