Full-length 16S analysis in Stroke and Sham mice.

Adult cerebral ischemia (stroke) is a neurological injury caused by the occlusion of cerebral blood vessels and it is one of the leading causes of long-term disability. Despite the large amount of evidence on adult stroke, very little is still known about the impact of a stroke occurring during perinatal ages in the developing brain. To address this question, we performed the middle cerebral artery occlusion at perinatal age (P14) in mice pups (MCAO mouse model of ischemic injury) and investigated the impact on motor and cognitive outcomes employing behavioral tests (gridwalk test, rotarod test, grip strength test, Y-maze test) longitudinally at different days (D) after stroke induction till adulthood. Full-length 16S ribosomal RNA gene form feces samples was also evaluated to understand if there was any difference in the microbiota composition of stroke mice than the sham one.