Finding genomic targets of transcription factor Six3 in neural stem cells.

Pro-neural transcription factor Six3 has been implicated in neuronal differentiation. We established primary neural stem cell with Six3 overexpression, and utilized CUT&RUN to profile genomic loci bound by Six3. Overall design: We cultured primary neural stem cells from mice and overexpressed Six3 in the cell lines. We differentiated the stem cells into neural lineage and performed CUT&RUN to profile genomic regions bound by Six3.