16S sequences from mesocosms experiment testing the effect of Siganus rivulatus on marine microorganisms

Nutrient cycling is a key biogeochemical process underlying the functioning of marine ecosystems. Yet, the contribution of fishes is still poorly understood. This is problematic considering the current modifications of fish assemblages experienced in certain regions such as the Mediterranean Sea, and their potential consequences in terms of ecosystem functioning. In this study, we used a mesocosm experiment to test the effect of nutrient recycling by an invasive marine herbivorous fish (Siganus rivulatus) on planktonic and benthic microbial communities. The response of these communities was assessed using a variety of analytical approaches such as measures of nutrient concentration, flow cytometry, and metabarcoding of the 16S rRNA gene. Our results show that several microbial compartments of marine ecosystems respond to the nutrients released by fish through excretion and egestion. The nutrients contained in the macroalgae consumed by S. rivulatus were excreted in large amounts as diss..., Experimental setup
The experiment aims to test the effect of nutrient recycling (excretion and egestion) by the invasive herbivorous fish Siganus rivulatus (Siganidae) on marine planktonic and benthic microbial communities. Eight 800L mesocosms were set up, which contained all the elements of natural marine ecosystems: sediment, macroalgae (4 species), rocks covered with turf and hiding places for fishes. Four mesocosm served as control and no fish were added while in the four treatment tanks we added three S. rivulatus individuals for a total biomass of 100g. The experiment lasted for eight days after which we sampled all the compartments of the system for analysis of their microbial communities (abundance, diversity) and their nutrient content.
DNA extraction
DNA extractions were performed in the molecular biology platforms of the MARBEC laboratory (UMR 9190, www.umr-marbec.fr) and at the Genseq platform (genseq.umontpellier.fr), using the Qiagen MagAttract PowerSoil DNA KF Kit, selec..., , # 16S sequences from mesocosms experiment testing the effect of Siganus rivulatus on marine microorganisms

The dataset contains 16s sequences describing the microbial communities from various compartments of mesocosms testing the effect of fishes on the environment.

## Description of the data and file structure

The file entitled 16S_sequences_mesocosm.zip contains the raw sequences obtained from sequencing of the bacterial 16S rRNA gene amplified from various compartment of mesocosms mimicking the marine environment of the Levantine Sea.

The file table_metadata.csv contains the metadata associated with the sequences. It contains the following columns:

* sample_id_fastq: the id of the sample in the siganidae_microbiome_16S_sequences.zip file
* barcoding_id: the id of the samples used for barcoding analyses
* sample_id: the unique sample ID
* taxonomy: the taxonomic identification of the sample
* sample_type: the type of sample categorized in broad categories
tank_id:...