SILAC APMS analysis of SRP72 interactome in U2OS cells

The Signal Recognition Particle (SRP) is a universally conserved and abundant ribonucleoprotein particle (RNP) in cells. Its composition and size vary across evolution. In mammals, SRP comprises one RNA molecule, the 7SL RNA, and six proteins: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72. SRP is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. To reveal novel aspects of SRP biogenesis, we established the interactome of the GFP-SRP72 protein. To do so, we have produced stable human U2OS cell line expressing GFP-SRP72 and performed stable isotope labeling by amino acids in cell culture (SILAC) proteomic experiment using GFP-SRP72 as bait.