CYP1A expression in freshwater fish of western New York as an indicator of pollution levels

Dataset collected: 12 freshwater fish species were collected from 7 sites in western New York, USA. using hoop nets Dataset processing: fish were humanely sacrificed (MS-222), tissues were extracted and presevered in RNA later and then analyzed using western blot analysis. CYP1A protein expression was measured using the CYP1A antibody (Caymen Chemical, USA; 173132) and protein samples were normalized to actin (Millipore, USA; MAB1501) and a loading control with positive CYP1A expression (BR2).