Evolutionarily conserved host factors required for <i>Brucella abortus</i> (S2308) infection in both <i>Drosophila</i> S2 and mammalian cell lines

aColony forming units (CFUs) of the untreated control was normalized as 100%. bData represent the means±standard deviations from at least three independent experiments. **represents significant at P<0.001 compared with no-RNAi control. cDominant-negative mutant. dNot detected. eData resulted from image analysis using NIH Image J software describe in Materials and Methods. fNo accurate data, ∼30% of infected cells with replicating bacteria. gMEFs are deficient in class IA PI3Ks p85α and p85β (p85α−/−p85β−/−). hDouble strand RNA targeting to the two PI3Ks Pi3K21B (CG2699) and Pi3K92E (CG4141) in class IA and IB, respectively. iDouble strand RNA targeting to all the four members of PI3Ks [Pi3K21B, Pi3K92E, Pi3K68D (CG11621, class II PI3K) and Pi3K59F (CG5373, class III PI3K)] in the fly genome.