Proteomic profiling of FBXO11 protein complexes

FBXO11, a member of the F-box protein family, plays a critical role in cellular processes such as protein degradation, cell cycle regulation, and signaling pathways by serving as a substrate recognition component of the SKP1-Cullin-F-box (SCF) ubiquitin ligase complex. Dysregulation of FBXO11 has been implicated in various diseases, including cancer and developmental disorders, yet its full spectrum of interactions and functional roles remains poorly understood. This project aims to perform a comprehensive proteomic profiling of FBXO11-associated protein complexes to uncover its interaction network and better understand its biological functions. Using advanced mass spectrometry-based proteomics, we will identify and characterize the proteins that interact with FBXO11 under different cellular conditions. Additionally, bioinformatics tools will be employed to analyze the identified interactome and explore their functional enrichment in specific pathways and biological processes.