Transcriptome analysis of SH-SY5Y and SK-N-AS neuroblastoma cells with KIT gene knockdown

The goal of this study was to identify dynamic changes in signaling pathway activities after KIT knockdown by RNAi in neuroblastoma with relatively high KIT expression (SH-SY5Y) and low expression (SK-N-AS). In two neuroblastoma cell line KIT expression was downregulated by shRNA lentiviral vector and transcriptome profiling was performed 3, 6 and 30 days after lentiviral transduction. Gene expression was normalized on neuroblastoma cell lines, transduced with control shRNA lentiviral vector.