A sex-specific trade-off between pesticide resistance and tolerance to heat-induced sterility in <i>Tetranychus urticae</i>

Summary:The data and scripts presented were used to assess the impact of high temperature and its interaction with pesticide resistance on three life-history traits (survival, developmental time and fertility) in the crop pest Tetranychus urticae. Firstly, using a susceptible population to etoxazole, we tested how different high temperatures affected female and male developmental time, the number of surviving juveniles, daily fecundity and offspring number, by exposing mites to either 25 (control), 33, 35, 36 or 37°C during their development. Then, we tested how temperature (25 or 36°C) and pesticide resistance (using susceptible and resistant populations) affected female and male developmental time, the number of surviving juveniles, offspring number and sex ratio. Files available:We provide 3 .R files (Script_statistical_analysis_high_temperatures, Script_statistical_analysis_interaction_temperature_pesticide_resistance, Script_figures) and 8 .csv files (Dataset_devtime_high_temperatures, Dataset_fert_high_temperatures, Dataset_devtime_interaction_temperature_pesticide_resistance, Dataset_fert_interaction_temperature_pesticide_resistance, Dataset_means_devtime_high_temperatures, Dataset_means_fert_high_temperatures, Dataset_means_devtime_interaction_temperature_pesticide_resistance, Dataset_means_fert_interaction_temperature_pesticide_resistance). Script_statistical_analysis_high_temperaturesThis file contains the script used to make the statistical analysis for the "Effect of high developmental temperatures on life-history traits" experiment. Script_statistical_analysis_interaction_temperature_pesticide_resistanceThis file contains the script used to make the statistical analysis for the "Interaction between pesticide resistance and response to high developmental temperature" experiment. Script_figuresThis file contains the script used to create figures 2 and 3. Dataset_devtime_high_temperaturesThis file contains the data of the effect of several high temperatures on developmental time and number of surviving juveniles, compared to the effect of the control temperature. Table headers are explained below: Temp - developmental temperature of the offspring (33, 35 or 36°C). Rep - replicate number per treatment. First_day_Adaughters - number of days the first female took to reach adulthood in a patch (i.e., replicate). M25First_day_Adaughters - average number of days the first female took to reach adulthood in a patch at 25°C. CompFirst_day_Adaughters - difference between First_day_Adaughters and M25First_day_Adaughters. First_day_Asons - number of days the first male took to reach adulthood in a patch (i.e., replicate). M25First_day_Asons - average number of days the first female took to reach adulthood in a patch at 25°C. CompFirst_day_Asons - difference between First_day_Asons and M25First_day_Asons. Total_adults - number of juveniles that survived until adulthood. M25Total_adults - average number of juveniles that survived until adulthood at 25°C. CompTotal_adults - difference between Total_adults and M25Total_adults. Fecundity - number of eggs growing in each patch. M25Fecundity - average number of eggs growing in each patch at 25°C. CompFecundity - difference between Fecundity and M25Fecundity. Dataset_fert_high_temperaturesThis file contains the data of the effect of several high temperatures on daily fecundity and offspring number, compared to the effect of the control temperature. Table headers are explained below: Temp_fxm - developmental temperatures of the female and male paired (♀33°C x ♂25°C, ♀35°C x ♂25°C, ♀36°C x ♂25°C, ♀37°C x ♂25°C). Temp_fem - developmental temperature of the female (33, 35, 36 or 37°C). Temp_male - developmental temperature of the male (25°C). Rep - replicate number per treatment. Daily_fec - mean number of eggs laid per day in each patch. M25Daily_fec - average mean number of eggs laid per day in each patch at 25°C. CompDaily_fec - difference between Daily_fec and M25Daily_fec. Total_adults - number of adult offspring. M25Total_adults - average number of adult offspring at 25°C. CompTotal_adults - difference between Total_adults and M25Total_adults. Dataset_devtime_interaction_temperature_pesticide_resistanceThis file contains the data of the effect of high temperature and pesticide resistance on developmental time and number of surviving juveniles. Table headers are explained below: Temp - developmental temperature of the offspring (25 or 36°C). Pop - resistance status of the individuals (WuSS - susceptible, WuRR - resistant). Rep - replicate number per treatment. Fecundity - number of eggs growing in each patch (i.e., replicate). First_day_Adaughters - number of days the first female took to reach adulthood in a patch. First_day_Asons - number of days the first male took to reach adulthood in a patch. Total_adults - number of juveniles that survived until adulthood. Observations - any observations made in each replicate. Dataset_fert_interaction_temperature_pesticide_resistanceThis file contains the data of the effect of high temperature and pesticide resistance on offspring number and offspring sex ratio. Table headers are explained below: Temp_fxm - developmental temperatures of the female and male paired (♀25°C x ♂25°C, ♀25°C x ♂36°C, ♀36°C x ♂25°C, ♀36°C x ♂36°C). Temp_fem - developmental temperature of the female (25 or 36°C). Temp_male - developmental temperature of the male (25 or 36°C). Pop - resistance status of the individuals (WuSS - susceptible, WuRR - resistant). Rep - replicate number per treatment. Age_male - male paired was taken from one of two cohorts, which have a difference of 1 day. Age_fem - female paired was taken from one of two cohorts, which have a difference of 1 day. Fecundity - number of eggs laid during 4 days per female. Total_daughters - number of adult daughters. Total_sons - number of adult sons. Total_adults - number of adult offspring. Sex_ratio - proportion of adult daughters in the offspring (adult daughters and sons). Observations - any observations made in each replicate. Dataset_means_devtime_high_temperaturesThis file contains the means of the effect of several high temperatures on developmental time and number of surviving juveniles, compared to the effect of the control temperature (in Dataset_devtime_high_temperatures) Table headers are explained below: Temp - developmental temperature of the offspring (33, 35 or 36°C). Mean_adults - mean difference between Total_adults and M25Total_adults. Mean_devtime_fem - mean difference between First_day_Adaughters and M25First_day_Adaughters. Mean_devtime_male - mean difference between First_day_Asons and M25First_day_Asons. Dataset_means_fert_high_temperaturesThis file contains the means of the effect of several high temperatures on daily fecundity and offspring number, compared to the effect of the control temperature (in Dataset_fert_high_temperatures) Table headers are explained below: Temp_fxm - developmental temperatures of the female and male paired (♀33°C x ♂25°C, ♀35°C x ♂25°C, ♀36°C x ♂25°C, ♀37°C x ♂25°C). Mean_dailyfec - mean difference between Daily_fec and M25Daily_fec. Mean_adults - mean difference between Total_adults and M25Total_adults. Dataset_means_devtime_interaction_temperature_pesticide_resistanceThis file contains the means of the effect of high temperature and pesticide resistance on developmental time and number of surviving juveniles. Table headers are explained below: Temp - developmental temperature of the offspring (25 or 36°C). Pop - resistance status of the individuals (WuSS - susceptible, WuRR - resistant). Mean_total_adults - mean number of juveniles that survived until adulthood. Mean_devtime_fem - mean number of days the first female took to reach adulthood in a patch. Mean_devtime_male - mean number of days the first male took to reach adulthood in a patch. Dataset_means_fert_interaction_temperature_pesticide_resistanceThis file contains the means of the effect of high temperature and pesticide resistance on offspring number and offspring sex ratio. Table headers are explained below: Temp_fxm - developmental temperatures of the female and male paired (♀25°C x ♂25°C, ♀25°C x ♂36°C, ♀36°C x ♂25°C, ♀36°C x ♂36°C). Temp_fem - developmental temperature of the female (25 or 36°C). Temp_male - developmental temperature of the male (25 or 36°C). Pop - resistance status of the individuals (WuSS - susceptible, WuRR - resistant). Mean_sexratio - mean proportion of adult daughters in the offspring (adult daughters and sons). Mean_totaladults - mean number of adult offspring.