Analysis of 3D human airway epithelial cultures by single-cell RNA-seq

We report the application of single-cell-based sequencing technology for high-throughput profiling of cell types and and transcriptional state of cells in the complex tissue of the human airway epithelium. Our model system is that of polarized human airway epithelial cultures, differentiated from hTert-immortalized basal-like precursor cells. Examination of single-cell transcriptomics in pseudostratified human airway epithelial cultures