Lentiviral barcode library sequencing - supplementary files

DNA sequencing to detect and analyse 30N barcodes of a lentiviral barcode library used for lineage tracing in mouse embryos. This information is used to flag barcodes that are over-represented in a library and could potentially confound clonal analysis. 

viral_R1.fastq.gz is a raw sequencing file of a PCR amplicon from a lentiviral prep. This file was processed in order to extract 30N barcodes from a specific location in the amplicon and generate a csv. file extracted_barcodes_viral.csv. This file also contains counts of how many times a specific barcode is found in the dataset. No correction was done for the barcode similarity (such as Hamming distance).

Scripts for barcode extraction are found at the associated github repository.