Expression data from the regeneration of the liver

In the partial liver resection and regeneration model, the early regeneration of the liver has strict gene regulation, and the regulation of liver genes is different in different resection proportions, which is a strictly controlled genetic procedure. Different genes are activated in liver resection of different proportions.For example, genes such as apoptotic autophagy inflammation were significantly expressed in 85% of liver resection cases. We used microarrays to describe in detail the global program of gene expression in regenerative livers and identify different classes of up-regulated or down-regulated genes in the process. We selected early regenerated livers for RNA extraction and hybridization on Affymetrix microarray.In order to improve the time resolution of the expression spectrum, we selected 3 same time points of the two excision models, namely 6h, 12h and 24h; three parallel samples were taken at each time point for analysis.