Gene expression of keratinocytes with miR-885-5p inhibited vs control

As miR-885-5p is a microRNA downregulated in Cutaneous Lupus Erythematosus (CLE) and it is localted in the epidemis and its function is focused in keratinocytes, human Arrays to study the role of miR-885-5p in Cutaneous Lupus Erythematosius will be conducted.Gene expression in keratinocytes transfected with anti-miR-885-5p will be studied. We have included 24 Samples: Non-stimulated control (4), Non-stimulated anti-miR-885-5p (4), UVB-stimulated control (4), UVB-stimulated antimiR-885-5p (4), IFN alpha-stimulated control (4) and IFN alpha-stimulated anti-miR-885-5p Microarray gene expression profiling. We will compare the gene expression of keratinocytes with miR-885-5p inhibited vs control. Keraitnocytes were cultured in 24 well plated and transfected with anti-miR-885-5p or anti-miRNA control according to standard protocols. 24h post-transfection, they will be stimulated with different inflammatory treatments: UVB 25 mJ/cm2, IFN alpha 10 ng/mL or PBS (control, non-stimualted). UVB and IFN alpha are known CLE triggers and revelant in the pathogenesis of CLE. Three comparisons will be analysed in order to understand the role of miR-885-5p: 1) Non-stimulated control vs Non-stimulated anti-miR 885-5p 2) UVB control vs UVB anti-miR-885-5p. 3) IFN alpha control vs IFN alpha anti-miR-885-5p.