In this study, we report the development of meticulous-integration Cas9 (miCas9) by fusing a minimal motif consisting of thirty-six amino acids encoded by BRCA2 Exon 27 (Brex27) to spCas9. In comparis

NIAID Data Ecosystem20

Oligonucleotides used to produce single guide RNA.

CRISPRi基因编辑

寡核苷酸合成

Oligonucleotides used to produce single guide RNA.

NIAID Data Ecosystem10

File S2.zip

CRISPRi基因编辑

基因敲入序列分析

Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.

Figshare2023-03-14 更新20

CRISPR RNA-seq from K562 (ENCSR929YET)

CRISPRi基因编辑

转录组分析

RNA-seq on K562 cells treated with a CRISPR gRNA against AIMP1. (AIMP1-BGKcLV26) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Re

NIAID Data Ecosystem10

Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing

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