Annex supplement

To further investigate the phylogenetic
position of A. japonica, complete chloroplast
genome sequences of this taxon and 14 other species selected from the Primulaceae
and one outgroup were used to construct phylogenetic tree. Camellia
granthamiana was selected as the outgroup of the phylogenetic tree. This
study also used 85 protein-coding genes to conduct a ML analysis under the
GTR + R + I model with 1000 bootstrap replicates. The phylogenetic tree showed
that A. japonica was clustered with
other species in Primulaceae with strong support (Figure 1). The complete
plastome sequence of A. japonica will
provide a useful resource for the conservation genetics of this species as well
as for the phylogenetic studies for Primulaceae.