Annex supplement

To further investigate the phylogenetic position of A. japonica, complete chloroplast genome sequences of this taxon and 14 other species selected from the Primulaceae and one outgroup were used to construct phylogenetic tree. Camellia granthamiana was selected as the outgroup of the phylogenetic tree. This study also used 85 protein-coding genes to conduct a ML analysis under the GTR + R + I model with 1000 bootstrap replicates. The phylogenetic tree showed that A. japonica was clustered with other species in Primulaceae with strong support (Figure 1). The complete plastome sequence of A. japonica will provide a useful resource for the conservation genetics of this species as well as for the phylogenetic studies for Primulaceae.