Stacks of microCT Scans, Cell size, weight, volume and thallus size data supporting the paper 'Mechanical regulation of tissue flatness in Marchantia'
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https://zenodo.org/record/13981437
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资源简介:
These data are the supporting elements to the following paper: 'Mechanical regulation of tissue flatness in Marchantia'
.tif files contain MicroCT (MCT) scans of 16-day-old Marchantia polymorpha thalli. Three genotypes were analysed here: fer-2 mutant (from Mecchia et al., 2022), FER-OE #9 (proMpEF1::MpFERONIA-mCitrine trangenic line 9) from Mecchia et al., 2022), and Tak-1 (WT line). These plants were grown in 3 different media: Gamborgh B5 + vitamins and 0.6, 1.2 and 2.5% agar, and one stress condition consisting of the adjunction of a thin PDMS film at 4, to mimich external mechanical stimulus (only performed on thalli grown on 1.2% agar).
MicroCT scans were performed at the faculity of odontology of Université Paris-Cité (Plateform imagerie du vivant) with the technical support of Lotfi Slimani and Baptiste Casel. https://piv.u-paris.fr/micro-ct-haute-resolution/
All files already have embeded scales.
Each file name consists of a unique ID number in the following form:
P++-
-LETTER: One letter = one imaging session
-NUMBER: Individual and Genotype: 33-40 -> Tak1; 200-207->fer-2; 41-49 -> FER-OE
-CONDITION : AGAR0.6/AGAR2.5/PDMS. Absence of condition indicates growth on standard medium (1.2% agar). PDMS indicated growth on standard medium and supplementation of a topping PDMS film at day 4)
-Volume data were calculated from MicroCT scans
-thallus projected surfaces were calculated from MicroCT scans
-Lambda curvature calculation.ipynb is suited for MorphographX mesh exported .txt files.
创建时间:
2024-10-25



