The 2014 and 2015 International Geobiology Courses took place at Little Hot Creek (LHC), California. The data shown here represents an attempt to gather information from a number of sources at LHC. Students will analyze the data and produce meaningful conclusions to the data presented using an integrated approach combining the expertise of geologists and biologists.. International GeoBiology Course 2014/2015

Samples were collected from a series of hot springs at the headwaters of Little Hot Creek located in the Long Valley Caldera near Mammoth Lake, CA. The springs are supersaturated for carbonate, circumneutral in pH, and range from a temperature of 50˚ to 80˚ C. Genomic DNA was isolated from biofilms and water filtered through a PES 0.22µm 13mm filter using the Zymo Xpedition Soil/Fecal kit (Zymo Research Corp., Irvine, CA). Sequencing was conducted using either MiSeq PE250, PE300, or HiSeq Rapid PE250. MiSeq runs were prepared using the Agilent SureSelect kit (Agilent Technologies, Irvine, CA) with an approximate insert size of 400bp, and HiSeq PE250 samples were prepared using the Nextera XT library preparation kit (Illumina, San Diego, CA).