Effect of Itape stripping in skin gene expression [TS_skin]

Mechanical skin injury promote an acute inflammation and promote the development of a dominated type 2 immune response. The mechanisms initiated by skin damage are not fully understood Overall design: After shaving the back of Balb/c mice was left intact or tape stripped 6 times with the help of a tegaderm. After 6h skin biopsy were collected and samples were stored in RNAlater. Total skin RNA was isolated with RNeasy Mini Kit (Qiagen), followed by cDNA synthesis using the SuperScript VILO cDNA Synthesis Kit (ThermoFisher Scientific). The Ion AmpliSeq Transcriptome Mouse Gene Expression Kit was used to prepare bar-coded libraries and sequenced by using an Ion S5 next-generation sequencer. The AmpliSeqRNA plug-in (ThermoFisher Scientific) was used to calculate differential gene expression analysis