Synthesis of 2'-formamidonucleoside phosphoramidites for suppressing the seed-based off-target effects of siRNAs

To investigate effects of siRNA with 2'-formamidonucleoside phosphoramidites (2'-FA) modifications on genome-wide off-target, we analyzed gene expression profile of HeLa cells transfected by their siRNAs. Gene expression profiling Total RNAs were extracted from HeLa cells transfected by mock, unmodified, or 2'-formamidonucleoside phosphoramidites (2'-FA) modified siRNA and performed microarray analysis.